Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F10%3A00354019" target="_blank" >RIV/67985939:_____/10:00354019 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/10:00354019
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae
Original language description
We applied CLSM and Two-Photon Excitation (TPE) microscopy techniques to visualize testate amoebae (TA), their test, morphology and physiology. TA are usually examined using SEM and E-SEM. It is not possible to acquire images of living TA and physiologyof the amoeba inside the test by SEM or E-SEM. The goal of the study was to examine the potential of CLSM and TPE for imaging of TA. We tried 17 fluorescent dyes to label different structures of TA. CLSM enabled us to acquire images of TA from depths upto 40 ?m, whereas TPE was able to penetrate to 60 ?m. Stereological methods were employed to estimate the volume of the biomass of TA visualized by CLSM
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy and Microanalysis
ISSN
1431-9276
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
Suppl.2
Country of publishing house
US - UNITED STATES
Number of pages
2
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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