Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications forTaxonomy and Ecophysiology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F10%3A00172888" target="_blank" >RIV/68407700:21460/10:00172888 - isvavai.cz</a>

Alternative codes found

RIV/67985939:_____/10:00354405 RIV/67985823:_____/10:00354405

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications forTaxonomy and Ecophysiology

Original language description

Testate amoebae (TA) are a group of free-living protozoa, important in ecology and paleoecology. TA taxonomy is mainly based on the morphological features of the shell, as examined by means of light microscopy or scanning electron microscopy (SEM/ESEM).We explored the potential applications of confocal laser scanning microscopy (CLSM), two photon excitation microscopy (TPEM), phase contrast, differential interference contrast (DIC Nomarski), and polarization microscopy to visualize TA shells and innerstructures of living cells, which is not possible by SEM or environmental SEM. Images captured by CLSM and TPEM were utilized to create three-dimensional (3D) visualizations and to evaluate biovolume inside the shell by stereological methods, to assess the function of TA in ecosystems. This approach broadens the understanding of TA cell and shell morphology, and inner structures including organelles and endosymbionts, with potential implications in taxonomy and ecophysiology.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EG - Zoology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

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UT code for WoS article

000284715300010

EID of the result in the Scopus database

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