A Novel Experimental Model of Acute Respiratory Distress Syndrome in Pig
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F16%3A10334326" target="_blank" >RIV/00064165:_____/16:10334326 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/16:10334326
Result on the web
<a href="http://www.biomed.cas.cz/physiolres/pdf/65/65_S643.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/65/65_S643.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
A Novel Experimental Model of Acute Respiratory Distress Syndrome in Pig
Original language description
Acute respiratory distress syndrome (ARDS) is severe medical condition occurring in critically ill patients and with mortality of 33-52 % is one of the leading causes of death in critically ill patients. To better understand pathophysiology of ARDS and to verify novel therapeutical approaches a reliable animal model is needed. Therefore we have developed modified lavage model of ARDS in the pig. After premedication (ketamine and midazolam) 35 healthy pigs were anesthetized (propofol, midazolam, morphin, pipecuronium) and orotracheally intubated and ventilated. Primary ARDS was induced by repeated cycles of lung lavage with a detergent Triton X100 diluted in saline (0.03 %) heated to 37 degrees C preceded by pre-oxygenation with 100 % O-2. Single cycle included two subsequent lavages followed by detergent suction. Each cycle was followed by hemodynamic and ventilation stabilization for approx. 15 min, with eventual administration of vasopressors according to an arterial blood pressure. The lavage procedure was repeated until the paO(2)/FiO(2) index after stabilization remained below 100 at PEEP 5 cm H2O. In 33 pigs we have achieved the desired degree of severe ARDS (PaO2/FiO(2)<100). Typical number of lavages was 2-3 (min.1, max. 5). Hemodynamic tolerance and the need for vasopressors were strongly individual. In remaining two animals an unmanageable hypotension developed. For other subjects the experimental ARDS stability was good and allowed reliable measurement for more than 10 h. The present model of the ARDS is clinically relevant and thus it is suitable for further research of the pathophysiology and management of this serious medical condition.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
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Volume of the periodical
65
Issue of the periodical within the volume
Supplement 5
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
"S643"-"S651"
UT code for WoS article
000392030300012
EID of the result in the Scopus database
2-s2.0-85009966189