The Influence of 7-OH Methotrexate Metabolite on Clinical Relevance of Methotrexate Determination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F11%3A7117" target="_blank" >RIV/00064203:_____/11:7117 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/11:7117 RIV/68407700:21460/11:00228143
Result on the web
<a href="http://www.ncbi.nlm.nih.gov/pubmed/21888024" target="_blank" >http://www.ncbi.nlm.nih.gov/pubmed/21888024</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The Influence of 7-OH Methotrexate Metabolite on Clinical Relevance of Methotrexate Determination
Original language description
Background: A modified high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of methotrexate (MTX) and its main metabolite 7-hydroxymethotrexate (7-OHMTX) and compared to the immunochemical fluorescence polarization immunoassay (FPIA2) determination of methotrexate. Methods: Methotrexate was determined by HPLC with UV detection at 303 nm after precipitation of proteins with trichloroacetic acid. Fluorescence polarization immunoassays (FPIA2) of methotrexate were performed on the TDx FLx Immunoassay Analyzer. Results: Our data indicate good correlation between methotrexate levels >1 mu mol/L determined by HPLC and FPIA2. (r = 0.94, Spearman correlation coefficient). However, concentrations of methotrexate <1 mu mol/L measured by fluorescence polarization immunoassay were overestimated. Conclusions: The concentration of MTX <1 mu mol/L are overestimated due to the cross reactivity with metabolites 7-OHMTX and 2,4-diamino-N(10)-methylp
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FS - Medical facilities, apparatus and equipment
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Laboratory
ISSN
1433-6510
e-ISSN
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Volume of the periodical
57
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
599-606
UT code for WoS article
000295251000017
EID of the result in the Scopus database
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