Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F22%3A10157311" target="_blank" >RIV/00098892:_____/22:10157311 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/chapter/10.1007/978-3-031-14627-5_39" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-14627-5_39</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-3-031-14627-5_39" target="_blank" >10.1007/978-3-031-14627-5_39</a>
Alternative languages
Result language
angličtina
Original language name
Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay
Original language description
The wound healing assay is one of the most widely used methods for studying cell migration and motility in vitro. In addition, modern automated equipment designed for time-lapse microscopy of living cells allows image sequences to be taken at short time intervals, enabling even more precise recording of cell migration dynamics. This paper describes the application of the procedure to evaluate image data acquired during wound healing of human chondrocytes and synovial sarcoma cells that were affected by the addition of synovial fluid from patients suffering from osteoarthritis. The proposed procedure is suitable for the analysis of wound healing assays even when the wound boundary cannot be detected by traditional methods, e.g. due to low cell density, numerous impurities, or when the distribution of cells forming the monolayer is affected by various factors present in the culture medium.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
<a href="/en/project/NU20-06-00269" target="_blank" >NU20-06-00269: Utility of cellular profiles and proteomics of synovial fluid and periprosthetic tissues for clinical decision making in knee osteoarthritis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Advances in Intelligent Networking and Collaborative Systems
ISBN
978-3-031-14626-8
ISSN
2367-3370
e-ISSN
2367-3389
Number of pages
14
Pages from-to
380-392
Publisher name
Springer Cham
Place of publication
Cham
Event location
Nishinomiya
Event date
Sep 7, 2022
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000870692600039