Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73618019" target="_blank" >RIV/61989592:15110/22:73618019 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/chapter/10.1007/978-3-031-14627-5_39" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-14627-5_39</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-3-031-14627-5_39" target="_blank" >10.1007/978-3-031-14627-5_39</a>
Alternative languages
Result language
angličtina
Original language name
Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay
Original language description
The wound healing assay is one of the most widely used methods forstudying cell migration and motility in vitro. In addition, modern automated equipmentdesigned for time-lapse microscopy of living cells allows image sequences tobe taken at short time intervals, enabling even more precise recording of cell migrationdynamics. This paper describes the application of the procedure to evaluateimage data acquired during wound healing of human chondrocytes and synovialsarcoma cells that were affected by the addition of synovial fluid from patients sufferingfrom osteoarthritis. The proposed procedure is suitable for the analysis ofwound healing assays even when the wound boundary cannot be detected by traditionalmethods, e.g. due to low cell density, numerous impurities, or when thedistribution of cells forming the monolayer is affected by various factors present inthe culture medium.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
<a href="/en/project/NU20-06-00269" target="_blank" >NU20-06-00269: Utility of cellular profiles and proteomics of synovial fluid and periprosthetic tissues for clinical decision making in knee osteoarthritis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Lecture Notes in Networks and Systems
ISBN
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ISSN
2367-3370
e-ISSN
2367-3389
Number of pages
14
Pages from-to
380-392
Publisher name
Polish Academy of Sciences
Place of publication
Polsko
Event location
Sanda-Shi, Japonsko
Event date
Sep 7, 2022
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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