Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F24%3A00080274" target="_blank" >RIV/00159816:_____/24:00080274 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/24:00136606 RIV/65269705:_____/24:00080274

Result on the web

<a href="https://www.nature.com/articles/s41598-024-67157-9" target="_blank" >https://www.nature.com/articles/s41598-024-67157-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-024-67157-9" target="_blank" >10.1038/s41598-024-67157-9</a>

Result language

angličtina

Original language name

Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts

Original language description

Nickel(Ni)-containing materials have been widely used in a wide range of medical applications, including orthopaedics. Despite their excellent properties, there is still a problem with the release of nickel ions into the patient&apos;s body, which can cause changes in the behaviour of surrounding cells and tissues. This study aims to evaluate the effects of Ni on bone cells with an emphasis on the determination of Ni localization in cellular compartments in time. For these purposes, one of the most suitable models for studying the effects induced by metal implants was used-the patient&apos;s osteoarthritic cells. Thanks to this it was possible to simulate the pathophysiological conditions in the patient&apos;s body, as well as to evaluate the response of the cells which come into direct contact with the material after the implantation of the joint replacement. The largest differences in cell viability, proliferation and cell cycle changes occurred between Ni 0.5 mM and 1 mM concentrations. Time-dependent localization of Ni in cells showed that there is a continuous transport of Ni ions between the nucleus and the cytoplasm, as well as between the cell and the environment. Moreover, osteoarthritic osteoblasts showed faster changes in concentration and ability to accumulate more Ni, especially in the nucleus, than physiological osteoblasts. The differences in Ni accumulation process explains the higher sensitivity of patient osteoblasts to Ni and may be crucial in further studies of implant-derived cytotoxic effects. (C) The Author(s) 2024.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30211 - Orthopaedics

Project

<a href="/en/project/NU20-08-00149" target="_blank" >NU20-08-00149: Multicentric evaluation of hypersensitivity reactions in patients indicated for total joint replacement including evaluation of the reasons for reimplanting</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

14

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

15

Pages from-to

16133

UT code for WoS article

001270360800063

EID of the result in the Scopus database

2-s2.0-85198385821