Intracellular distribution of the ?Np73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F13%3A%230000406" target="_blank" >RIV/00209805:_____/13:#0000406 - isvavai.cz</a>

Result on the web

<a href="http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm" target="_blank" >http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Intracellular distribution of the ?Np73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Original language description

The p73 protein is a member of the p53 family of transcription factors that has two N-terminal isoforms: the TAp73 isoform is reported to have a tumor suppressor function, whereas the ?Np73 isoform likely has oncogenic potential. The expression of theseisoforms and the differences in their intracellular distribution have been described in many cancer types; however, little is known about the p73 isoforms in brain tumors. Our study is focused on the intracellular localization of ?Np73 in medulloblastomacell lines. Due to a lack of suitable anti-?Np73 antibodies, we developed two new rabbit polyclonal antibodies, ?Np73-26 and ?Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Bothof these new antibodies were subsequently used for analysis of the ?Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear loca

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histology and Histopathology

ISSN

0213-3911

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

7

Country of publishing house

ES - SPAIN

Number of pages

12

Pages from-to

913-924

UT code for WoS article

000320710900011

EID of the result in the Scopus database

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