Overexpression of the Delta Np73 isoform is associated with centrosome amplification in brain tumor cell lines

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F15%3A00063592" target="_blank" >RIV/65269705:_____/15:00063592 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/15:00084860

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs13277-015-3474-3" target="_blank" >http://link.springer.com/article/10.1007%2Fs13277-015-3474-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13277-015-3474-3" target="_blank" >10.1007/s13277-015-3474-3</a>

Result language

angličtina

Original language name

Overexpression of the Delta Np73 isoform is associated with centrosome amplification in brain tumor cell lines

Original language description

The p73 protein is a member of the p53 family, and this protein is known to be essential for the maintenance of genomic stability, DNA repair, and apoptosis regulation. Transcription from two promoters leads to two main N-terminal isoforms: the TAp73 isoform is reported to have tumor suppressor function, whereas the Delta Np73 isoform likely has oncogenic potential. The present study is focused on the investigation of a possible role of both these p73 N-terminal isoforms in the process of centrosome amplification. HGG-02 and GM7 glioblastoma cell lines and the Daoy medulloblastoma cell line were used in this study. The cells were analyzed using indirect immunofluorescence to determine TAp73 and Delta Np73 expression patterns and possible co-localization with the BubR1 protein, as well as the number of centrosomes. A transiently transfected GM7 cell line was used to verify the results concerning the N-terminal isoforms in relation to centrosome amplification. We found that increased immunoreactivity for the Delta Np73 isoform is associated with the occurrence of an abnormal number of centrosomes in particular cells. Using the transiently transfected GM7 cell line, we confirmed that centrosome amplification is present in cells with overexpression of the Delta Np73 isoform. In contrast, the immunoreactivity for the TAp73 isoform was weak or medium in most of the cells with an aberrant number of centrosomes. To determine the putative counterpart of the p73 N-terminal isoforms among spindle assembly checkpoint (SAC) proteins, we also evaluated possible interactions between the N-terminal isoforms and BubR1 protein, but no co-localization of these proteins was observed.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/EE2.3.20.0183" target="_blank" >EE2.3.20.0183: Center of Experimental Biomedicine</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Tumor Biology

ISSN

1010-4283

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

10

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

7483-7491

UT code for WoS article

000362969900018

EID of the result in the Scopus database

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