Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00065656" target="_blank" >RIV/00216224:14310/13:00065656 - isvavai.cz</a>

Result on the web

<a href="http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm" target="_blank" >http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Original language description

This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficientspecificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/NS10218" target="_blank" >NS10218: The role of p73 protein isoforms in medulloblastoma pathogenesis: an immunocytochemical and microarray study</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histology and Histopathology

ISSN

0213-3911

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

7

Country of publishing house

ES - SPAIN

Number of pages

12

Pages from-to

913-924

UT code for WoS article

000320710900011

EID of the result in the Scopus database

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