The use of capillary electrophoresis with contactless conductivity detection for sensitive determination of stevioside and rebaudioside A in foods and beverages
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F17%3A43912258" target="_blank" >RIV/00216208:11120/17:43912258 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.foodchem.2016.09.135" target="_blank" >http://dx.doi.org/10.1016/j.foodchem.2016.09.135</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodchem.2016.09.135" target="_blank" >10.1016/j.foodchem.2016.09.135</a>
Alternative languages
Result language
angličtina
Original language name
The use of capillary electrophoresis with contactless conductivity detection for sensitive determination of stevioside and rebaudioside A in foods and beverages
Original language description
Two electrophoretic methods with contactless conductivity detection have been developed for determination of the content of rebaudioside A and stevioside in samples of sweeteners and beverages prepared from extracts of the plant Stevia rebaudiana Bertoni. The total content of rebaudioside A and stevioside can be determined in a fused silica capillary with an inner diameter of 10 μm and total length of 31.5 cm in optimised background electrolyte with the composition 170 mM H3BO3/LiOH (pH 9.0). The combined peak of the two glucosides is characterised by a migration time of 54 s, which completely separates it from EOF. INST coating solution in an amount of 0.5% v/v, which effectively suppresses the electroosmotic flow, was added to the background electrolyte for mutual separation of rebaudioside A and stevioside. The CE method with suppression of EOF is characterised by complete separation of rebaudioside A and stevioside, LOD is 0.3 mg/L (0.1 μM).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA15-03139S" target="_blank" >GA15-03139S: New Electrophoretic Approaches in Studies of Obesity and Diabetes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food Chemistry
ISSN
0308-8146
e-ISSN
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Volume of the periodical
219
Issue of the periodical within the volume
March
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
193-198
UT code for WoS article
000386984100025
EID of the result in the Scopus database
2-s2.0-84988553095