Expression profiles of iron transport molecules along the duodenum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F22%3A43923388" target="_blank" >RIV/00216208:11120/22:43923388 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/22:10443142 RIV/00179906:_____/22:10443142
Result on the web
<a href="https://doi.org/10.1111/jcmm.17313" target="_blank" >https://doi.org/10.1111/jcmm.17313</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jcmm.17313" target="_blank" >10.1111/jcmm.17313</a>
Alternative languages
Result language
angličtina
Original language name
Expression profiles of iron transport molecules along the duodenum
Original language description
Duodenal biopsies are considered a suitable source of enterocytes for studies of dietary iron absorption. However, the expression level of molecules involved in iron absorption may vary along the length of duodenum. We aimed to determine whether the expression of molecules involved in the absorption of heme and non-heme iron differs depending on the location in the duodenum. Analysis was performed with samples of duodenal biopsies from 10 individuals with normal iron metabolism. Samples were collected at the following locations: (a) immediately post-bulbar, (b) 1-2 cm below the papilla of Vater and (c) in the distal duodenum. The gene expression was analyzed at the mRNA and protein level using real-time PCR and Western blot analysis. At the mRNA level, significantly different expression of HCP1, DMT1, ferroportin and Zip8 was found at individual positions of duodenum. Position-dependent expression of other molecules, especially of FLVCR1, HMOX1 and HMOX2 was also detected but with no statistical significances. At the protein level, we observed statistically significantly decreasing expression of transporters HCP1, FLVCR1, DMT1, ferroportin, Zip14 and Zip8 with advancing positions of duodenum. Our results are consistent with a gradient of diminishing iron absorption along the duodenum for both heme and non-heme iron.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cellular and Molecular Medicine
ISSN
1582-1838
e-ISSN
1582-4934
Volume of the periodical
26
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
2995-3004
UT code for WoS article
000784089600001
EID of the result in the Scopus database
2-s2.0-85128395321