Methods to assess DC-dependent priming of T cell responses by dying cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10410922" target="_blank" >RIV/00216208:11130/20:10410922 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/20:10410922
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=J93ELXLp72" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=J93ELXLp72</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/bs.mie.2019.05.045" target="_blank" >10.1016/bs.mie.2019.05.045</a>
Alternative languages
Result language
angličtina
Original language name
Methods to assess DC-dependent priming of T cell responses by dying cells
Original language description
Dendritic cells have been widely investigated in cancer immunotherapy clinical trials for the last two decades mainly due to their robust ability to elicit an adaptive anticancer immune response of the cellular and humoral types. Immature DCs can be easily loaded with desired antigens. However, to become efficient antigen-presenting cells, DCs must first undergo a process of maturation. Protocols for the generation of DCs for use in cancer immunotherapy, including the generation of a large number of immature DCs for antigen pulsing and the selection of a well-defined immunostimulatory agent to achieve complete and reproducible maturation, which is a crucial step for further stimulation of T cell activation, must carefully consider the characteristics of DC physiology. In this report, we provided a detailed protocol for DC generation, pulsation and activation with the subsequent induction of T cell-specific immune responses. (C) 2020 Elsevier Inc.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Enzymology
ISSN
0076-6879
e-ISSN
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Volume of the periodical
632
Issue of the periodical within the volume
Cellular Methods Pt B
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
55-65
UT code for WoS article
000556278200006
EID of the result in the Scopus database
2-s2.0-85068227265