Mitoxantrone in Combination with a DNA-PK Inhibitor: Possible Therapy of Promyelocytic Leukaemia Resistant Forms

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10106825" target="_blank" >RIV/00216208:11150/11:10106825 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/11:00002579

Result on the web

<a href="http://fb.cuni.cz/file/5600/FB2011A0029.pdf" target="_blank" >http://fb.cuni.cz/file/5600/FB2011A0029.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mitoxantrone in Combination with a DNA-PK Inhibitor: Possible Therapy of Promyelocytic Leukaemia Resistant Forms

Original language description

The aim of the study was to sensitize cells of human promyelocytic leukaemia HL-60/MX2 (resistant to mitoxantrone and further substances interacting with topoisomerase II - TI II) to the effect of mitoxantrone (MTX). We demonstrated that the main mechanism of the HL-60/MX2 cell atypical multiple drug resistance is not only their altered activity of TI II and reduced levels of TI II ? and ?. The resistance of the HL-60/MX2 cells to MTX is associated with their increased ability to repair DSB. The HL-60/MX2 cells, compared to HL-60 cells (which are MTX-sensitive), contain large amounts of DNA-PK, which is responsible for the main pathway of the DSB repair, non-homogenous end joining (NHEJ), and they also contain large amounts of further repair proteins Rad50 and Nbs1, which are important in both NHEJ and homologous re-combination. We demonstrated that specific DNA-PK inhibitor NU7026 in HL60/MX2 prevented DSB repair through the NHEJ pathway and essentially abolished the resistance to MTX

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5500

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

200-205

UT code for WoS article

000297182800004

EID of the result in the Scopus database

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