HPLC determination of arginases inhibitor N-(omega)-hydroxy-nor-L-arginine using core-shell particle column and LC-MS/MS identification of principal metabolite in rat plasma

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10105402" target="_blank" >RIV/00216208:11150/12:10105402 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/12:10105402
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1570023211007537" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1570023211007537</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jchromb.2011.11.022" target="_blank" >10.1016/j.jchromb.2011.11.022</a>

Result language
angličtina
Original language name
HPLC determination of arginases inhibitor N-(omega)-hydroxy-nor-L-arginine using core-shell particle column and LC-MS/MS identification of principal metabolite in rat plasma
Original language description
For the purpose of in vivo pharmacokinetic studies, an HPLC method was developed and validated for the quantification of N-(omega)-hydroxy-nor-L-arginine, L-arginine and N-(omega)-ethyl-L-arginine (internal standard) in rat plasma. Sample processing involved a solid-phase extraction on the Waters MCX cartridges and on-line pre-column derivatization of the analytes with o-phthaldialdehyde and 3-mercaptopropionic acid. Separation of the derivatives was carried out on a core-shell Kinetex C18 column in a gradient elution mode with a mobile phase consisting of methanol and water (pH = 3.00 adjusted with formic acid). Fluorimetric detection with the excitation/emission wavelengths of 235/450 nm was used. The method was validated according to the FDA guidelines and applied to pilot pharmacokinetic experiments. An unknown metabolite was extracted from the plasma of Wistar rats after a single bolus of N-(omega)-hydroxy-nor-L-arginine (iv. 10 mg kg(-1)). The metabolite was identified as nor-L-a
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN
1570-0232
e-ISSN
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Volume of the periodical
880
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
90-99
UT code for WoS article
000299197600013
EID of the result in the Scopus database
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