Flubendazole induces mitotic catastrophe and senescence in colon cancer cells in vitro

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F16%3A10322146" target="_blank" >RIV/00216208:11150/16:10322146 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/16:10322146
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/jphp.12503/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jphp.12503/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jphp.12503" target="_blank" >10.1111/jphp.12503</a>

Result language
angličtina
Original language name
Flubendazole induces mitotic catastrophe and senescence in colon cancer cells in vitro
Original language description
Objectives: Flubendazole (FLU), a member of benzimidazole family of anthelmintic drugs, is able to inhibit proliferation of various cancer cells. The aim of present study was to elucidate the mechanisms of antiproliferative effect of FLU on colorectal cancer cells in vitro. Methods: The effect of FLU on proliferation, microtubular network, DNA content, caspase activation and senescence induction was studied in SW480 and SW620 cell lines. Key findings: Flubendazole significantly affected cell proliferation in a pattern typical for mitotic inhibitor. This was accompanied by decrease in cyclin D1 levels, increase in cyclin B1 levels, activation of caspase 2 and caspase 3/7 and PARP cleavage. Morphological observations revealed disruption of microtubular network, irregular mitotic spindles, formation of giant multinucleated cells and increase in nuclear area and DNA content. In SW620 cell line, 37.5% giant multinucleated cells induced by FLU treatment showed positivity for SA-beta-galactosidase staining. Cell lines were able to recover from the treatment and this process was faster in SW480 cells. Conclusion: Flubendazole in low concentration temporarily inhibits cell proliferation and induces mitotic catastrophe and premature senescence in human colon cancer cells in vitro.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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