Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126406" target="_blank" >RIV/00216208:11310/12:10126406 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-012-6140-2" target="_blank" >http://dx.doi.org/10.1007/s00216-012-6140-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-012-6140-2" target="_blank" >10.1007/s00216-012-6140-2</a>

Result language

angličtina

Original language name

Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode

Original language description

A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantif

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

404

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

693-699

UT code for WoS article

000307251900011

EID of the result in the Scopus database

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