Application of Flow Cytometry for Genome Size Determination in Geosmithia Fungi: A Comparison of Methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10289248" target="_blank" >RIV/00216208:11310/14:10289248 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/14:00436051
Result on the web
<a href="http://dx.doi.org/10.1002/cyto.a.22500" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22500</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.22500" target="_blank" >10.1002/cyto.a.22500</a>
Alternative languages
Result language
angličtina
Original language name
Application of Flow Cytometry for Genome Size Determination in Geosmithia Fungi: A Comparison of Methods
Original language description
Genome size has played an important role in the evolution of plants and animals because changes in genome size seem to accompany if not facilitate evolutionary adaptation to environmental conditions. Flow cytometry (FCM) is a widespread method for determining genome size thanks to its high accuracy and speed of measurements. Nevertheless, only a few comparative studies of FCM methods exist in the field of mycology, and reviews are absent. In this study, we compared the suitability of several concentrations and RNAse A incubation times, fixatives and buffers for estimating genome size in fungi. We chose the genus Geosmithia as a model filamentous fungus. We also introduced a new standard, Aspergillus fumigatus CEA10, to determine absolute genome size. We found FCM to be an appropriate method for measuring genome size in fungi, but optimization steps showed that incorrect propidium iodide staining of nuclei can overestimate genome size due to cytoplasmic staining. We identified fixation
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytometry Part A
ISSN
1552-4922
e-ISSN
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Volume of the periodical
85A
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
854-861
UT code for WoS article
000342636200005
EID of the result in the Scopus database
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