Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10293513" target="_blank" >RIV/00216208:11310/15:10293513 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/15:10293513 RIV/00064165:_____/15:10293513
Result on the web
<a href="http://dx.doi.org/10.1016/j.clinbiochem.2014.12.002" target="_blank" >http://dx.doi.org/10.1016/j.clinbiochem.2014.12.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.clinbiochem.2014.12.002" target="_blank" >10.1016/j.clinbiochem.2014.12.002</a>
Alternative languages
Result language
angličtina
Original language name
Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS
Original language description
Objectives: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicologypurposes. Design and methods: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. Results: The assay was proved to be quadratic in the range of 50 to 5000 mg L-1 with adequate accuracy (96.3-105.8%) and precision (CV {= 8.9%). Conclusion: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R-2 0.9933 for ethylene glycol and 0.9943 for glycolic acid). (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Biochemistry
ISSN
0009-9120
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
3
Country of publishing house
CA - CANADA
Number of pages
3
Pages from-to
189-191
UT code for WoS article
000348703300019
EID of the result in the Scopus database
2-s2.0-84919499914