Behaviour and detection of acridine-type DNA intercalators in urine using an electrochemical DNA-based biosensor with the protective polyvinyl alcohol membrane

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10378555" target="_blank" >RIV/00216208:11310/18:10378555 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.jelechem.2017.11.028" target="_blank" >https://doi.org/10.1016/j.jelechem.2017.11.028</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2017.11.028" target="_blank" >10.1016/j.jelechem.2017.11.028</a>

Result language
angličtina
Original language name
Behaviour and detection of acridine-type DNA intercalators in urine using an electrochemical DNA-based biosensor with the protective polyvinyl alcohol membrane
Original language description
The aim of this work is preparation and use of an electrochemical DNA-based biosensor with a textured surface for the investigation of dsDNA host-guest interactions with specific chemicals in a biological sample of complex matrix such as urine. Results obtained at the search and optimization of polymer protective films over the DNA recognition layer at a glassy carbon electrode show that a polyvinyl alcohol membrane is the effective barrier able to stabilize the biosensor response in urine. The detection of acridines using the square-wave voltammetric (SWV) scan in the cathodic direction has allowed overcoming a problem of the electrochemical interference of DNA bases and acridines at the direct anodic scan. Height of the well-separated SWV peak at around 0.00 V to + 0.10 V (which corresponds to the reduction of the oxidation products of both of acridines) depends on the concentration of acridines. The peak potentials of acridines were shifted by about 0.10 V to the negative potential values in the urine medium compared to phosphate buffer. Intercalation of acridine yellow and proflavine into dsDNA was confirmed both in phosphate buffer and in urine.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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