Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10395083" target="_blank" >RIV/00216208:11310/19:10395083 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=kEUVkey1bp" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=kEUVkey1bp</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/s19092103" target="_blank" >10.3390/s19092103</a>
Alternative languages
Result language
angličtina
Original language name
Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis
Original language description
A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold-carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IUmL(-1) to 1600 IUmL(-1), with a detection limit of 50 IUmL(-1). The coefficient of determination (r(2)) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA17-03868S" target="_blank" >GA17-03868S: New Methods of Electrochemical Monitoring of Biologically Active Organic Compounds in Environmental, Biological and Food Matrices</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Sensors
ISSN
1424-8220
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
2103
UT code for WoS article
000469766800147
EID of the result in the Scopus database
2-s2.0-85065758889