Electrochemical immunoassay for the detection of antibodies to tick-borne encephalitis virus by using various types of bioconjugates based on silver nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43920986" target="_blank" >RIV/60461373:22310/20:43920986 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10418225
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S1567539420300256?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1567539420300256?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bioelechem.2020.107576" target="_blank" >10.1016/j.bioelechem.2020.107576</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical immunoassay for the detection of antibodies to tick-borne encephalitis virus by using various types of bioconjugates based on silver nanoparticles
Original language description
This work reports for the first time a significantly improved and simplified electrochemical immunoassay to detect antibodies to tick-borne encephalitis virus (TBEV) using a 96-well microtiter plate as a platform for immobilization and silver nanoparticles (AgNPs) as electrochemical labels. The electrochemical assay is performed by detecting the elemental silver oxidation signal where the electroactive signalling silver species are released from the bioconjugates (Ab@AgNP, AbS@AgNP, and ProteinA@AgNP). For this purpose, AgNPs were synthesized and further tagged with biomolecules (antibodies to TBEV, cleaved antibodies to TBEV, and protein A). Signal is read by linear sweep anodic stripping voltammetry (LSASV) of silver ions (through the electrochemical stripping of accumulated elemental silver) on a graphite electrode (GE). AbS@AgNP was chosen as the best option for the new electrochemical immunoassay. The results of electrochemical measurements demonstrated that voltammetric signal increased with the increasing concentration of target antibodies to TBEV within the range from 100 to 1600 IU mL(-1), with a detection limit of 90 IU mL(-1). To verify the practical application of the novel electrochemical immunosensor, the quantity of immunoglobulins against TBEV in human serum was checked. The results may contribute to the development of alternative methods for monitoring TBEV in biological fluids. (C) 2020 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GC20-01417J" target="_blank" >GC20-01417J: New approaches preventing passivation in monitoring of environmental organic pollutants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioelektrochemistry
ISSN
1567-5394
e-ISSN
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Volume of the periodical
135
Issue of the periodical within the volume
october
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
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UT code for WoS article
000579730600015
EID of the result in the Scopus database
2-s2.0-85085658511