Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemicals Immunoassay of Tick-Borne Encephalitis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918679" target="_blank" >RIV/60461373:22310/19:43918679 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1424-8220/19/9/2103" target="_blank" >https://www.mdpi.com/1424-8220/19/9/2103</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s19092103" target="_blank" >10.3390/s19092103</a>

Result language

angličtina

Original language name

Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemicals Immunoassay of Tick-Borne Encephalitis

Original language description

A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL−1 to 1600 IU·mL−1, with a detection limit of 50 IU·mL−1 . The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

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UT code for WoS article

000469766800147

EID of the result in the Scopus database

2-s2.0-85065758889