Electrochemical immunoassay of antibodies using freshly prepared and aged conjugates of silver nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10472865" target="_blank" >RIV/00216208:11310/23:10472865 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=vxxOzhsZ1r" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=vxxOzhsZ1r</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2022.124028" target="_blank" >10.1016/j.talanta.2022.124028</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical immunoassay of antibodies using freshly prepared and aged conjugates of silver nanoparticles
Original language description
In this work, silver nanoparticles (AgNPs) were conjugated with immunoglobulins G using a simple passive adsorption method and the composition and properties of freshly prepared (Ab@AgNPs) and aged (Ab@AgNP/ Ag2SNPs) conjugates were investigated for the first time by transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS) and voltammetry. It was found that the state of the AgNPs in the conjugates changed during storage. In the freshly prepared Ab@AgNPs conjugates, silver is present in the metallic state only, while the aged Ab@AgNP/Ag2SNPs conjugates are characterized by the presence of a metallic core of AgNPs coated with a "corona" of Ag2S. This affects the selection of voltammetric conditions for recording an analytical signal from the silver label in the conjugates. The Ab@AgNPs and Ab@AgNP/Ag2SNPs conjugates were successfully tested in the electrochemical immunoassay of dog immuno-globulins as a model. A solid-phase indirect non-competitive assay was performed using a 96-well ELISA plate. To record intense analytical signals from the silver conjugates by linear scan anodic stripping voltammetry (LSASV) on a carbon composite electrode (CCE), they were preliminarily dissolved in acidic solutions. In the case of the Ab@AgNPs conjugate, a nitric acid solution (1 mol L(-1)) was used to dissolve silver conjugates, while in the case of Ab@AgNP/Ag2SNPs, a 1:1 mixture of 1 mol L(-1) CH3COOH and 1 mol L(-1) HNO3 was used. The CH3COOH dissolved the Ag2S "corona", and HNO3 dissolved the silver core of the Ab@AgNP/Ag2SNPs conjugate.This work opens up the possibility of the prolonged use of silver conjugates in electrochemical immunoassay, which can be used in commercial test systems for immunological studies as an alternative to conjugates labelled with enzyme labels.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GC20-01417J" target="_blank" >GC20-01417J: New approaches preventing passivation in monitoring of environmental organic pollutants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Volume of the periodical
253
Issue of the periodical within the volume
February
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
124028
UT code for WoS article
000916658400006
EID of the result in the Scopus database
2-s2.0-85142166488