Method for evaluation of ionic interactions in liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10416653" target="_blank" >RIV/00216208:11310/20:10416653 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=x9JIar7PU1" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=x9JIar7PU1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2020.461301" target="_blank" >10.1016/j.chroma.2020.461301</a>
Alternative languages
Result language
angličtina
Original language name
Method for evaluation of ionic interactions in liquid chromatography
Original language description
In this work we utilized basic and acidic analytes to investigate the ionic interaction participation in retention behavior of selected reversed-phase and polar columns. The test analytes included nitrate, benzenesulfonate and trimethylphenylammonium ions. The fully aqueous mobile phase comprising 10 mM dichloroacetic acid buffered with ammonia solution to desirable pH was used for retention experiments. Developed method was utilized to study the ionic interactions of stationary phases in pH range between 2.5 and 9.0. We demonstrate that selected sorbents used for reversed-phase and hydrophilic interaction chromatography separations exhibit cation- or anion-exchange interactions. We compare the results to novel Atlantis PREMIER BEH C18 AX mixed-mode column that combines reversed-phase and anion-exchange interaction modes. We evaluated the relative retention strength of selected columns for anionic and cationic analytes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA20-19655S" target="_blank" >GA20-19655S: Strategy for on-line protein digestion followed by separation in mixed-mode chromatography</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Volume of the periodical
1625
Issue of the periodical within the volume
August
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
461301
UT code for WoS article
000552017600038
EID of the result in the Scopus database
2-s2.0-85086145823