A novel method for characterisation of ionic properties of stationary phases using a buffer blending by the pumps
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10502183" target="_blank" >RIV/00216208:11310/25:10502183 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=aaG3ausuTL" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=aaG3ausuTL</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2025.114913" target="_blank" >10.1016/j.microc.2025.114913</a>
Alternative languages
Result language
angličtina
Original language name
A novel method for characterisation of ionic properties of stationary phases using a buffer blending by the pumps
Original language description
Mixed mode chromatographic columns are used for complex mixture separation. However, the ligand information of many commercially sold columns, such as the pKa of its functional group, is unknown, which complicates the method development. We propose a novel method for ionic interaction evaluation, designed for stationary phase characterisation. The method uses buffer blending by pumps to produce a range of mobile phase pH within the liquid chromatography system. The applicability of the method was utilised for ionic property elucidation of mixed mode stationary phases of weak anion-exchange character (column Acclaim WAX-1) and weak cation-exchange character (column Acclaim WCX-1). The interaction mechanism of these mixed mode columns was investigated using pump blended mobile phases in the 2.5-8.6 pH range, and observing the retention of strong acid/base analytes retention response to the dissociation/protonation of the ionic groups present on the stationary phase. This method was useful to determine the pKa value of the stationary phase ligand.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchemical Journal
ISSN
0026-265X
e-ISSN
1095-9149
Volume of the periodical
217
Issue of the periodical within the volume
October
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
114913
UT code for WoS article
001562290300001
EID of the result in the Scopus database
2-s2.0-105013631807