Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F02%3A00006844" target="_blank" >RIV/00216224:14110/02:00006844 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve
Original language description
Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. Thedorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellularconditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 ľm) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Imm
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Cellular and extracellular environment for the axon regeneration following injury of the spinal roots and peripheral nerves</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Progress in Basic, Applied, and Diagnostic Histochemistry
ISBN
80-210-2998-6
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
16
Publisher name
Masarykova univerzita v Brně
Place of publication
Brno
Event location
2002, Brno
Event date
Jan 1, 2002
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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