Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00144394" target="_blank" >RIV/00216224:14110/25:00144394 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10514065
Result on the web
<a href="https://www.nature.com/articles/s41598-025-08626-7" target="_blank" >https://www.nature.com/articles/s41598-025-08626-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-025-08626-7" target="_blank" >10.1038/s41598-025-08626-7</a>

Result language
angličtina
Original language name
Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions
Original language description
Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1 Delta background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA23-05284S" target="_blank" >GA23-05284S: Deciphering the relationship between homologous recombination and gene silencing</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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