High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00015575" target="_blank" >RIV/00216224:14310/06:00015575 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser

Original language description

A high-throughput axial MALDI-TOF mass spectrometer utilizing a laser with a 2 kHz pulse repetition-rate was constructed and tested. This fast mass spectrometer provided a data acquisition rate 10 times faster than a commercially available (200 Hz) axialmass spectrometer, while maintaining comparable limits of detection (200 amole of Glu fib peptide). Mass resolution, only slightly less than the commercial instrument (10,000 vs. 14,000), was sufficient for baseline resolution of isotopic clusters of peptides with m/z &lt;2700. A new method of mass calibration, which combined a limited number of internal and external standards, provided the same 15 ppm mass accuracy over the entire sample plate on either instrument. Implementing the 2 kHz laser required a faster data acquisition system and high-voltage pulse electronics, together with a novel strategy for rapid sample plate movements during acquisition, to achieve a sample analysis rate of up to 2 spots per second (with 800 shots/spot)

Czech name

Vysoce výkonná axiální MALDI-TOF MS s 2 kHz laserem

Czech description

Byl zkonstruován a vyzkoušen vysoce výkonný axiální MALDI-TOF MS s 2 kHz laserem. Detekční limit cca 200 amole of Glu fib peptidu, hmotnostní rozlišení 10000, správnost stanovení m/z 15 ppm, rychlost analýzy až 2 vzorky za sekundu při 800 pulsech na vzorek. Demonstrace analýzy 650 frakcí z LC-MS během 12 minut. Další detaily v anglické anotaci.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F03%2F0515" target="_blank" >GA203/03/0515: Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Chemistry

ISSN

0003-270

e-ISSN

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Volume of the periodical

78

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

912-919

UT code for WoS article

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EID of the result in the Scopus database

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