Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00021741" target="_blank" >RIV/00216224:14310/07:00021741 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/07:#0000200

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor

Original language description

The piezoelectric sensor (quartz crystal microbalance, QCM) was used to monitor cell adhesion in real time. Two cell lines, rat epithelial cells (WB F344) and lung melanoma cells (B16F10) were used. The cells were adhered and grown on the gold surface ofthe sensor pre-coated with adsorbed layer of extracellular matrix proteins as vitronectin and laminin. The process of cell attachment and spreading on the gold surface was continuously monitored and displayed by changes of the resonant frequency f and resistance R values of the piezoelectric resonators. The initial phase of cell attachment and spreading induced a decrease of frequency and increase of resistance relating viscoelastic properties of the cell monolayer on the sensing surface. The steady-state of both shifts was achieved after a few hours. The presence and state of cells on the surface was confirmed by fluorescent microscopy. The obtained results demonstrate that the piezoelectric sensor is suitable for studies of the cell

Czech name

Adheze eukaryotickych buecnych linii na povrch zlata modifikovany extracelularnimi bunecnymi proteiny

Czech description

Pomoci piezoelektrickeho biosensoru byla studovana adheze bunecnych linii na povrch zlata modifikovany vhodnymi proteiny.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biosensors & Bioelectronics

ISSN

0956-5663

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

9-10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

1896-1901

UT code for WoS article

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EID of the result in the Scopus database

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