Application of the (GTG)5-PCR fingerprinting for rapid identification of staphylococcal species occuring in humans.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026049" target="_blank" >RIV/00216224:14310/08:00026049 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Application of the (GTG)5-PCR fingerprinting for rapid identification of staphylococcal species occuring in humans.
Original language description
Objectives Staphylococci are natural inhabitants of skin, glands and mucous membranes of mammals and birds and play very important role in human infections as well. Development and evaluation of effective and reliable identification tools is needed for routine and rapid identification of increasing number of Staphylococcus spp. occurring in human clinical material. Method A group of 68 type and reference staphylococcal strains representing 22 taxa of human associated staphylococci was analyzed by rep-PCR fingerprinting with the (GTG)5 primer. The clinical isolates as well as strains isolated from healthy hosts and environmental samples were tested. Results All tested strains were typeable by the (GTG)5-PCR producing DNA fragments ranging from 200 bp to4500 bp. Majority of the tested species revealed species-specific fingerprints.
Czech name
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Czech description
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Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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