ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00052407" target="_blank" >RIV/00216224:14310/11:00052407 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES
Original language description
The protonation equilibria1 of short-chain cytosine homo-oligodeoxyribonucleotides (ODNs) were studied by means of voltammetry and UV-Vis spectra. The work was aimed at ssDNA fragments with different length of the ODN chain. The shortest and longest ODNswere dC3 and dC18, respectively. In order to know the thermodynamic stability of ODNs, the effect of temperature and ionic strength was studied. The protonation constants were calculated by using a hard model (OPIUM or EQUISPEC) 2,3. It was found that the prolongation of the ODN chain supports the formation of more non-canonical bonds in the hemiprotonated (C+-C) pair (?i-motif?). While the exothermic protonation of nucleotides (CMP or dCMP) does not differ, the thermodynamic parameters change for ODNsas a consequence of the existence of an ?i-motif? confirmed also by elimination voltammetry with linear scan (EVLS)4. These facts are important for the knowledge of the secondary structure of ODNs containing C nucleobase.
Czech name
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Czech description
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Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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