APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00052423" target="_blank" >RIV/00216224:14310/11:00052423 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
Original language description
Biosensors based on DNA or RNA aptamers, represent a very efficient platform for protein recognition1. We developed a novel label-free aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein complexformation using gold CD-trodes (GCDT). GCDT are electrodes fabricated from recordable compact discs that contain a fine gold layer on their surface. Low-cost GCDTs present good versatility and acceptable reproducibility. The thrombin biosensor is constructed by self-assembling a thiol-modified aptamer onto a GCDT surface, followed by recognition of the protein via monitoring the interfacial electron transfer resistance in the presence of an [Fe(CN)6]3-/4- redox couple2,3. The aptasensor was also characterized by cyclic voltammetry and the individual binding steps were analysed by scanning electron microscopy.
Czech name
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Czech description
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Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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