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GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00060052" target="_blank" >RIV/00216224:14310/12:00060052 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION

  • Original language description

    In this work, we report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance spectroscopy (EIS) upon aptamer-protein formation at the surface of gold CDtrodes (GCDTs) in the presence of redox couple [Fe(CN)6]3-/4-[2]. The biosensor was constructed by self-assembling a thiol-modified thrombin binding aptamer (TBA) onto the GCDT surface followed by the protein addition in a wide range of concentrations ranging from 20 nM to 1 microM. Achieved results reveal that the GCDTs present good versatility and suitable reproducibility for thrombin recognition with a limit of detection (LOD) of 5 nM. Non-specific interaction with similar molecular compounds like bovine serum albumin (BSA) demonstrates an appropriate and specifically target sensor response thanks to the aptamer incorporation as transducer elements. In order to verify the practical application of GCDT aptasensor in complex biological fluids, we have detected thrombin in spiked diluted blood p

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    XII. Pracovní setkání fyzikálních chemiků a elektrochemiků

  • ISBN

    9788073756185

  • ISSN

  • e-ISSN

  • Number of pages

    2

  • Pages from-to

    28-29

  • Publisher name

    Mendelova univerzita v Brně

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Jan 1, 2012

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article