Multimode fibre: a pathway towards deep tissue fluorescence microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00116094" target="_blank" >RIV/00216224:14310/15:00116094 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1117/12.2202355" target="_blank" >https://doi.org/10.1117/12.2202355</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2202355" target="_blank" >10.1117/12.2202355</a>
Alternative languages
Result language
angličtina
Original language name
Multimode fibre: a pathway towards deep tissue fluorescence microscopy
Original language description
Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS
ISBN
9781628418903
ISSN
0277-786X
e-ISSN
1996-756X
Number of pages
6
Pages from-to
1-6
Publisher name
SPIE-INT SOC OPTICAL ENGINEERING
Place of publication
BELLINGHAM
Event location
Sydney, AUSTRALIA
Event date
Dec 6, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000370723500052