High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00497594" target="_blank" >RIV/68081731:_____/18:00497594 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41377-018-0094-x" target="_blank" >http://dx.doi.org/10.1038/s41377-018-0094-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41377-018-0094-x" target="_blank" >10.1038/s41377-018-0094-x</a>

Result language
angličtina
Original language name
High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging
Original language description
Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 +/- 0.04 mu m across a 50-mu m field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
<a href="/en/project/EF15_003%2F0000476" target="_blank" >EF15_003/0000476: Holographic endoscopy for in vivo applications</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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