On-Line Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 inhibitors

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00113958" target="_blank" >RIV/00216224:14310/20:00113958 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_11" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0163-1_11" target="_blank" >10.1007/978-1-0716-0163-1_11</a>

Result language
angličtina
Original language name
On-Line Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 inhibitors
Original language description
The market share of single-enantiomer drugs is steadily increasing. The pharmacodynamics and pharmacokinetics of individual enantiomers can differ considerably. Thus, their characteristics have to be addressed as early as possible in the development process of new pharmaceuticals. Capillary electrophoresis is a promising technique for enantioselective drug metabolism studies due to highly effective separations, minuscule consumption of sample and reagents, compatibility with a variety of detection techniques, high-throughput via automation, and the implementation of on-line procedures. An on-line method comprised of the diffusion-based mixing of cytochrome P450 3A4 with racemic ketamine, incubation of the enzyme reaction, separation of the reaction products S- and R-norketamine and their quantification is presented in this chapter. Since diffusion is an inherent property of all molecules, the method enables the addition of virtually any compound to the reaction mixture without the need for additional optimization of the mixing conditions, and thus can be favorably used for the rapid screening of putative cytochrome P450 3A4 inhibitors.
Czech name
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Czech description
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Project
<a href="/en/project/GA16-06106S" target="_blank" >GA16-06106S: Capillary electrophoresis based high-throughput system for beta-secretase inhibitor screening as a therapeutic target of Alzheimer's disease</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
Targeting Enzymes for Pharmaceutical Development: Methods and Protocols
ISBN
9781071601624
Number of pages of the result
12
Pages from-to
167-178
Number of pages of the book
278
Publisher name
Springer
Place of publication
USA
UT code for WoS chapter
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