Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00077479" target="_blank" >RIV/00216224:14740/14:00077479 - isvavai.cz</a>
Result on the web
<a href="http://www.plantcell.org/content/26/5/2156" target="_blank" >http://www.plantcell.org/content/26/5/2156</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1105/tpc.114.126607" target="_blank" >10.1105/tpc.114.126607</a>
Alternative languages
Result language
angličtina
Original language name
Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid
Original language description
DNA double-strand break (DSB) repair mechanisms differ in their requirements for a homologous repair template and in the accuracy of the result. We aimed to quantify the outcome of repair of a single targeted DSB in somatic cells of young barley (Hordeumvulgare) plants. Amplicon sequencing of three reporter constructs revealed 47 to 58% of reads as repaired via nonhomologous end-joining (NHEJ) with deletions and/or small (1 to 3 bp) insertions. Alternative NHEJ revealed 2 to 5 bp microhomology (15.7% of cases) or new replication-mediated short duplications at sealed breaks. Although deletions outweigh insertions in barley, this bias was less pronounced and deleted sequences were shorter than in Arabidopsis thaliana. Between 17 and 33% of reads likelyrepresent restoration of the original sequence. Depending on the construct, 20 to 33% of reads arose via gene conversion (homologous recombination).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0189" target="_blank" >EE2.3.20.0189: Development of excellence in evolutionary cytogenomics, epigenetics and cell signalling</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The Plant Cell
ISSN
1040-4651
e-ISSN
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Volume of the periodical
26
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
2156-2167
UT code for WoS article
000338771700030
EID of the result in the Scopus database
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