Interaction kinetics reveal distinct properties of conformational ensembles of three-repeat and four-repeat tau proteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F22%3A00128730" target="_blank" >RIV/00216224:14740/22:00128730 - isvavai.cz</a>
Result on the web
<a href="https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14339" target="_blank" >https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14339</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/1873-3468.14339" target="_blank" >10.1002/1873-3468.14339</a>
Alternative languages
Result language
angličtina
Original language name
Interaction kinetics reveal distinct properties of conformational ensembles of three-repeat and four-repeat tau proteins
Original language description
Tau protein is an intrinsically disordered protein. Its physiological state is best described as a conformational ensemble (CE) of metastable structures interconverting on the local and molecular scale. The monoclonal antibody DC39C recognizes a linear C-terminal tau epitope, and as the tau interaction partner, its binding parameters report about tau CE. Association kinetics of DC39C binding, together with crosslinking mass spectrometry, show differences in the accessibility of the C terminus in CEs of tau isoforms. Furthermore, removal of the C terminus accelerated the aggregation kinetics of three-repeat tau proteins. Our results suggest a novel mechanism of splicing-driven regulation of the tau C-terminal domain with consequences on the specific roles of tau isoforms in microtubule assembly and pathological aggregation. Keywords
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
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Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEBS Letters
ISSN
0014-5793
e-ISSN
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Volume of the periodical
596
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1178-1189
UT code for WoS article
000774661700001
EID of the result in the Scopus database
2-s2.0-85127351596