Occurence of Fusarium in food and feed

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002650" target="_blank" >RIV/00216275:25310/05:00002650 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Occurence of Fusarium in food and feed
Original language description
Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory medi
Czech name
Plísně rodu Fusarium v potravinách a krmivech
Czech description
určeno pro seznam publikací

Project
<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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