Detection of toxigenic fusarium from foods by PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00005992" target="_blank" >RIV/00216275:25310/07:00005992 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of toxigenic fusarium from foods by PCR

Original language description

Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 bp. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphological features are important for species classification where the PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. Thus the PCR technique has proved

Czech name

Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR

Czech description

Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Papers of the University of Pardubice, Series A

ISSN

1211-5541

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

13

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

12

Pages from-to

73-85

UT code for WoS article

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EID of the result in the Scopus database

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