Toxigenic Fusarium in Food and Feed
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00004294" target="_blank" >RIV/00216275:25310/06:00004294 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Toxigenic Fusarium in Food and Feed
Original language description
Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals.Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff.
Czech name
Toxinogenní fusária v potravinách a krmivech.
Czech description
určeno pro seznam publikací
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
20th International ICFMH Symposium Food Micro 2006
ISBN
961-90346-3-5
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
412
Publisher name
International Committee on Food Microbiology and Hygiene
Place of publication
Bologna, Itálie
Event location
Bologna
Event date
Aug 29, 2006
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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