Výskyt toxinogenních plísní rodu Fusarium v potravinách

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00005888" target="_blank" >RIV/00216275:25310/06:00005888 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

čeština

Original language name

Výskyt toxinogenních plísní rodu Fusarium v potravinách

Original language description

PCR procedure according to Edwards et al. (2001) was optimised with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F?683 and selectivity was examinedusing reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non?toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2?3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.

Czech name

Výskyt toxinogenních plísní rodu Fusarium v potravinách

Czech description

PCR procedure according to Edwards et al. (2001) was optimised with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F?683 and selectivity was examinedusing reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non?toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2?3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.

Type

D - Article in proceedings

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník Mikrobiologie potravin

ISBN

80-7080-625-7

ISSN

—

e-ISSN

—

Number of pages

6

Pages from-to

93-98

Publisher name

Vysoká škola chemicko-technologická v Praze

Place of publication

Praha

Event location

—

Event date

—

Type of event by nationality

—

UT code for WoS article

—