New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896446" target="_blank" >RIV/00216275:25310/13:39896446 - isvavai.cz</a>
Result on the web
<a href="http://www.znaturforsch.com/ac/v68c/c68c.htm#No3" target="_blank" >http://www.znaturforsch.com/ac/v68c/c68c.htm#No3</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors
Original language description
A new and simple analytical method is described for the determination of the IC50 values of the inhibitors of the hydrolysis of acetylcholine (ACh) or acetylthiocholine (ATCh) by cholinesterases. The method is based on monitoring the time course of the pH value during the uninhibited and inhibited reaction. It requires only a pH meter with a suitable pH measuring cell and a small thermostated stirred batch reactor. The method has been validated for twelve different types of cholinesterase inhibitors. The determined IC50 values are comparable to those obtained by independent, more complicated, and expensive methods (Ellman's and pH-stat).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Zeitschrift fuer Naturforschung C-A Journal of Biosciences
ISSN
0939-5075
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
3-4
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
133-138
UT code for WoS article
000322090700009
EID of the result in the Scopus database
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