PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39897856" target="_blank" >RIV/00216275:25310/14:39897856 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/14:00436781
Result on the web
<a href="http://dx.doi.org/10.1016/j.msec.2014.04.011" target="_blank" >http://dx.doi.org/10.1016/j.msec.2014.04.011</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.msec.2014.04.011" target="_blank" >10.1016/j.msec.2014.04.011</a>
Alternative languages
Result language
angličtina
Original language name
PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays
Original language description
In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly( ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC). Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, andbioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres. Both neat and PEGylated microspheres were then incubated with the inert protein bovine serum albumin or cells to evaluatethe rate of nonspecific adsorption (NSA). PEG with Mr of 30,000 Da was responsible for 45% reduction in NSA of proteins and 74% for cells compared to neat particles. The microspheres' behavior in PDMS and COC microchannels was then evalu
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials Science & Engineering C: Materials for Biological Applications
ISSN
0928-4931
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
July
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
308-315
UT code for WoS article
000338388400041
EID of the result in the Scopus database
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