New Method for Automated Disk Diffusion Test

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F14%3APU110974" target="_blank" >RIV/00216305:26220/14:PU110974 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/14:00507186

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

New Method for Automated Disk Diffusion Test

Original language description

Microbial resistence to antibiotics is a very important parameter in the selection of a proper therapy and in the control of resistence spreading. One of the most used methods for measuring microbial susceptibility is the disk diffusion test. The test is based on diffusion of concentrated antibiotics from paper disk into agar. Concentration of antibiotics in the agar is dependent on distance from the center of the disc. Near antibiotic disc is concentration much higher due difficult permeation of antibiotics in agar. The diffused antibiotics then inhibit growth of sensitive strains. The zones are usually measured manually or by electronic calipers. Several aproaches for automatic inhibition zone detection have been introduced in recent years. Nevertheless, most of the algorithms are based on a similar radial profile analysis. We have designed a novel image-processing algorithm for measuring the size of inhibition zones of antibiotics based on an analysis of corrected image and rated this image with multicriterial algorithms (based on an observation of the radius profile). The algorithm was tested on 100 clinical isolates, resulting in calculation accuracy of 89% (ratio of success computed radii). If we include alternative radii (to be selected manually), the precision of the calculation rises to 98% (tolerance deviations between manual and automatic measurements were 2 mm). The achieved accuracy was independent of the culture medium (e.g. Muller-Hinton, blood agar, chocolate agar). The main advantage of the algorithm is the invariantness to the tested bacterial strain and culture medium. The new algorithm offers an alternative way to determine inhibition zones and evaluate antimicrobial susceptibility.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of PIERS 2014 in Guangzhou

ISBN

978-1-934142-28-8

ISSN

1559-9450

e-ISSN

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Number of pages

3

Pages from-to

192-194

Publisher name

Neuveden

Place of publication

Guangzhou, Čína

Event location

Guangzhou

Event date

Aug 25, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000393225900037