New Method for Automated Disk Diffusion Test

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00507186" target="_blank" >RIV/68081731:_____/14:00507186 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/14:PU110974

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

New Method for Automated Disk Diffusion Test

Original language description

Microbial resistence to antibiotics is a very important parameter in the selection of a proper therapy and in the control of resistence spreading. One of the most used methods for measuring microbial susceptibility is the disk diffusion test. The test is based on diffusion of concentrated antibiotics from paper disk into agar. Concentration of antibiotics in the agar is dependent on distance from the center of the disc. Near antibiotic disc is concentration much higher due difficult permeation of antibiotics in agar. The diffused antibiotics then inhibit growth of sensitive strains. The zones are usually measured manually or by electronic calipers. Several aproaches for automatic inhibition zone detection have been introduced in recent years. Nevertheless, most of the algorithms are based on a similar radial profile analysis. We have designed a novel image-processing algorithm for measuring the size of inhibition zones of antibiotics based on an analysis of corrected image and rated this image with multicriterial algorithms (based on an observation of the radius profile). The algorithm was tested on 100 clinical isolates, resulting in calculation accuracy of 89% (ratio of success computed radii). If we include alternative radii (to be selected manually), the precision of the calculation rises to 98% (tolerance deviations between manual and automatic measurements were 2 mm). The achieved accuracy was independent of the culture medium (e.g., Muller-Hinton, blood agar, chocolate agar). The main advantage of the algorithm is the invariantness to the tested bacterial strain and culture medium. The new algorithm offers an alternative way to determine inhibition zones and evaluate antimicrobial susceptibility.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

20201 - Electrical and electronic engineering

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

PIERS 2014 Guangzhou Proceedings

ISBN

978-1-934142-28-8

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

192-194

Publisher name

The Electromagnetics Academy

Place of publication

Cambridge

Event location

Guangzhou

Event date

Aug 25, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000393225900037