Identification of viable lactobacillus cell in fermented dairy products
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F10%3APU86545" target="_blank" >RIV/00216305:26310/10:PU86545 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Identification of viable lactobacillus cell in fermented dairy products
Original language description
PCR in combination with ethidium monoazidem (EMA) is a new method that allows to distinguish between live and dead cells. Expand the use of PCR in food for example when determining the number of live and dead cells of probiotic bacteria in dairy products. The method is based on the observation that EMA can penetrate into the cytoplasm and the DNA of cells killed due to malfunction of the cell membrane (wall). Photoactivation with EMA covalently binds to DNA, which is why Does Not Amplify in PCR. The PCRamplified only the DNA of living cells. The aim was to use EMA-PCR method to distinguish between live and dead bacterial cells probiotic Lactobacillus paracasei ssp paracasei CCDM 211 / 06, newly isolated from human sources. Attention was paid to various procedures of killing bacterial cells.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B08070" target="_blank" >2B08070: Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sborník přednášek V. sympozia společnosti pro probiotika a prebiotika
ISBN
978-80-213-2072-7
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
16-16
Publisher name
ČZU v Praze
Place of publication
Praha
Event location
Praha
Event date
Apr 20, 2010
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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