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PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F11%3APU94888" target="_blank" >RIV/00216305:26310/11:PU94888 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

  • Original language description

    The aim of this study was to identify yeasts isolated from red wine of Rulandske modre variety (Vitis vinifera) from integrated and ecological agriculture mode (production) from South Moravia. The yeast strains were isolated in regular intervals during spontaneous fermented must (without any SO2 addition). Together, we identified 124 strains (60 from ecological; 64 from integrated fermented must). As a rapid identification method, we used PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) and by application of specific primers (ITS1-ITS4) we amplified 5.8S-ITS segment of rDNA. PCR-RFLP of ITS regions using HaeIII, HinfI, TaqI, AluI and MseI (TruI) was used for identification and grouping of genetically similar strains. Restriction fragments were detected by agarose gel electrophoresis. All obtained electrophoreograms were processed by software BioNumerics 6.5 and UPGMA cluster analysis.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů