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Use of PCR-DGGE for control of bacteria in chesses and their pickles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU112164" target="_blank" >RIV/00216305:26310/14:PU112164 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Use of PCR-DGGE for control of bacteria in chesses and their pickles

  • Original language description

    Denaturation gradient gel electrophoresis (PCR-DGGE) was used for control of bacteria in brained cheeses and their pickles. Composition of polymerase chain reaction (PCR) mixture and DNA amplification conditions were optimized for PCR with CG clamp primers. Phenol extraction and magnetic microspheres were used for purification of amplicons. Different numbers and intensities of amplicons reflect different bacterial composition in tested cheeses and pickles.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    N - Vyzkumna aktivita podporovana z neverejnych zdroju

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů